分子量:25.2 kDa

纯度:>92% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Kallikrein 4, His Tag by the LAL method.

Buffer:50 mM Tris, 150 mM NaCl,  pH 7.5

生物活性:Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. (R&D Systems, Catalog # ES011) The specific activity is >200 pmoles / min / μg.

产品特性:Human Kallikrein 4, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 25.2 kDa. The predicted N-terminus is Ser 27. The reducing (R) protein migrates as 30-32 kDa in SDS-PAGE  due to glycosylation.

产品背景:Kallikrein-4 (KLK4) is also known as Enamel matrix serine proteinase 1 (EMSP1), Kallikrein-like protein 1 (KLK-L1), Prostase, Serine protease 17 (PRSS17), PSTS, which belongs to the peptidase S1 family and Kallikrein subfamily. KLK4 contains one peptidase S1 domain. KLK4 is expressed in prostate and involved in enamel formation. Defects in Kallikrein-4 / KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) which is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions.